Welchrom 96-Well Plates: Versatile and Efficient

In the fields of biotechnology, drug development, and food safety, laboratory testing is an indispensable part. The 96-well plate, as a commonly used basic tool in laboratories, provides researchers with a convenient and efficient experimental platform. Let's learn more about it together!

1. Composition and Structure of the 96-Well Plate

● The 96-well plate is made of high-quality polystyrene material, which has good transparency and chemical stability, meeting various experimental needs.

● The "96-well" design makes it versatile in many scenarios!

For example:

1. Used for sample storage: It can replace traditional 1.5mL centrifuge tubes, allowing simultaneous storage of a large number of samples, saving space, and keeping the setup tidy.

2. Used for sampling: It can be directly placed into the sample chamber of an automatic sampler for sample injection. This not only doubles the sample volume in the injection chamber but also allows direct injection into the chamber, eliminating the hassle of repeatedly drawing samples, placing samples, capping, inserting inserts, and cleaning bottles.

3. Used for sample pretreatment: If we pre-fill the 96-well plate with solid-phase extraction (SPE) materials, the 96-well plate becomes a "mini SPE column that can preprocess 96 samples simultaneously." As many of you know, there are numerous types of SPE materials, so when combined with different materials, the 96-well plate gives rise to a myriad of application scenarios.

2. Advantages of the 96-Well Plate

1. High Throughput: The 96 wells of the 96-well plate allow multiple experiments to be conducted simultaneously, greatly improving experimental efficiency and saving time and labor costs.

2. Flexibility: The 96-well plate can be used for various types of experiments, such as enzyme-linked immunosorbent assay (ELISA), cell culture, molecular cloning, and more, meeting different experimental needs.

3. Ease of Operation: The design of the 96-well plate makes experimental operations simpler. There is no need for complex equipment; experiments can be performed with just a simple pipette.

4. High Data Accuracy: Each well of the 96-well plate has precise dimensions and depth, ensuring the accuracy and reproducibility of experimental results.

3. Application Fields of the 96-Well Plate

1. Biotechnology: In fields such as genetic engineering, protein research, and cell culture, the 96-well plate provides researchers with a convenient experimental platform.

2. Drug Development: In areas such as drug screening, efficacy evaluation, and drug metabolism, the 96-well plate offers efficient experimental methods for drug development personnel.

3. Food Safety: In aspects like food testing, pesticide residue analysis, and veterinary drug residue analysis, the 96-well plate provides reliable technical support for food safety testing.

4. Environmental Monitoring: In water quality testing, soil pollution analysis, and air pollution analysis, the 96-well plate serves as a convenient experimental tool for environmental monitoring personnel.

4. Case Study Sharing

1. Determination of Amitriptyline, Alprazolam, Betamethasone, and Phenacetin Residues in Plasma

Plasma samples are purified using Welchrom® BRP 96-well plates. The separation is performed using an Ultisil® XB-C18, 3μm, 2.1×100mm chromatography column. Quantitative analysis is conducted by mass spectrometry in positive ion mode using Multiple Reaction Monitoring (MRM).

Table 1.1 Spiked recovery rate table

2. Detection of 25-Hydroxyvitamin D content in serum

After the serum is purified using PPT 96-well protein precipitation plates and Welchrom® BRP 96-well plates, it is separated using an Ultisil® XB-C18, 3μm, 2.1×50mm chromatography column. Quantitative analysis is performed using mass spectrometry in positive ion mode with MRM (Multiple Reaction Monitoring).

Figure 2.1 Serum sample profile

Figure 2.2. Serum sample spiked chromatogram (concentration 100ng/mL)
Table 2.1 Spiked recovery rate table

3. Determination of catecholamines in plasma

After the plasma is purified using Welchrom® P-WCX 96-well plates, it is separated using an Ultisil® PFP, 3μm, 2.1×50mm chromatography column. Quantitative analysis is performed using mass spectrometry in positive ion mode with MRM (Multiple Reaction Monitoring).

Figure 3.1 Sample matrix standard 50ppb

Table 3.1 Spiked recovery rate table